The estimation of mucin in gastric juice.
نویسندگان
چکیده
The importance of mucin as a constituent of gastric mucus and of acid gastric juice has been generally recognized (2). Numerous methods for the quantitative determination of mu&n have been described, the estimation of the reducing power having been the method almost universally used. The viscosity, acid-combining power, nitrogen content, and iodinecombining power have each been considered as a measure of mucin concentration; methods based on these properties have been applied to the whole gastric secretion or its contents or to various fractions isolated by ultrafiltration, electrodialysis, and the use of various protein precipitants (3-8). However, none of these methods has gained general acceptance (2). Mucoitinsulfuric acid is regarded as a characteristic prosthetic group of gastric mucin and many other mucoproteins (9). It has been isolated from hog gastric mucus (10-13) and from pure canine gastric juice (14). Hexuronic acid (12) or, more specifically, glucuronic acid (9, 13) has been established as one of the four components of this polysaccharide. Since (gluc)uronic acid appears to be the most characteristic component of gastric mucin and several reliable methods for its estimation are available, we investigated the possibility of utilizing the estimation of glucuronic acid as a measure of the concentration of mucin in gastric juice. We have found that a modification of Tollens’ naphthoresorcinol reaction for glucuronic acid (15) results in a consistent recovery of uranic acid from gastric mucin, mucoitinsulfuric acid, and whole canine gastric juice or mucus.
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عنوان ژورنال:
- The Journal of biological chemistry
دوره 176 2 شماره
صفحات -
تاریخ انتشار 1948